UBPBio
UBPBio JavaScript seem to be disabled in your browser. You must have JavaScript enabled in your browser to utilize the functionality of this website. Magento Commerce My Account Log In My Cart Home ProductsRELAY TR-FRET Reagents & KitsProteasomes (Proteins/Kits/Antibodies)E1 Activating EnzymesE2 Conjugating EnzymesE3 Ubiquitin LigasesUbiquitin / Sumo ChainsUbiquitin / Ubiquitin-like Proteins / MutantsDeubiquitinating EnzymesInhibitorsFluorescent Peptide / Protein SubstratesUbiquitin Binding ProteinsUbiquitin / UBL DerivativesApoptosis / NecrosisAntibodiesTag/Loading ControlProteasomesE1 / E1-like ProteinsUb / Ub-like ProteinsE3 Ub LigasesDUBsInflammasomesE2 Ub-Conjugating EnzymesAntibody Sampler KitsKitsProtein Expression / Purification ReagentsDNA / Protein LaddersCovid-19 Research Services Service/Tech Support Order Center Distributors Resources Search: ProductsRELAYTR FRET Reagents & KitsProteasomes / Activators / SubunitsE1 Activating EnzymesE2 Conjugating Enzymes E3 Ubiquitin Ligases Polyubiquitin / PolySUMO ChainsUbiquitin / Ubiquitin-like Proteins / MutantsDeubiquitinating EnzymesInhibitors Fluorescent Peptide / Protein SubstratesUbiquitin Binding ProteinsUbiquitin / UBL DerivativesApoptosis / Necrosis AntibodiesKitsProtein Expression / Purification ReagentsDNA / Protein Ladders Covid-19 ResearchCustom Research ServicesOur custom research services are supported by extensive technical expertise and hands-on experience.Protein Production Recombinant protein expression in E. coli, insect, or mammalian systems, followed by multi-step purification to achieve high purity and functional integrity.TR-FRET Reagents, Assays, and Research Services Development and execution of High-throughput TR-FRET–based assays using covalent modifiers compatible with terbium/fluorescein and europium/Cy5 donor–acceptor pairs. Custom Ubiquitin Research Tailored biochemical studies designed to interrogate the ubiquitin-proteasome system, compound screening, and drug target validation.Submit service request with a brief description of your project objectives and technical requirements. RELAYTR FRET PlatformFluorescence Resonance Energy Transfer (FRET) is a powerful technique used to detect molecular proximity between a donor and an acceptor fluorophore within a distance of 1–10 nm. In time-resolved FRET (TR-FRET) assays, the long fluorescence lifetime of lanthanides (such as terbium and europium) enables delayed signal detection, effectively eliminating short-lived background fluorescence. This time-resolved measurement significantly enhances assay sensitivity and specificity. Our RELAYTR FRET Platform leverages terbium and europium chelate-based donors paired with fluorescein- and Cy5-based acceptors. These fluorophores can be covalently conjugated to targets via free thiol or amine groups, ensuring robust and reliable performance and versative assay formats for detecting molecular interactions, post-translational modifications, enzyme activity, protein abundance, and more.We provide both ready-to-use reagents and customized CRO services to support diverse research and development needs. E2 Screening and Profiling Kits29 recombinant E2 enzymesE2 Screening Kit – gel-based AssayIdentifying E2s for E3sAssessing E2 activityE2 Profiling Kit – RELAYTR FRET AssayMonitoring E2-Ub charging (schematic below)High sensitivity – detecting 10 nM E2 activityHigh signal to background (S/B) ratio Profiling 23 ubiquitin E2sHigh throughput compatibleRELAYTR FRET Assay - E2-Ub ChargingDegrader Drug DiscoveryReagents and KitsTerbium, europium, fluorescein or Cy5 labeled ubiquitin, protein G, and anti-6xHis, GST, HA or DYKDDDDK antibody. View all reagents. CRO ServicesReagent production – terbium/europium/dye labelingAssay development and research serviceUb Ligase Activity Assay KitKey FeaturesDetecting Ub ligase autoubiquitination High throughput TR-FRET assay enabled with terbium-/fluorescein-ubiquitinExcellent signal to background (S/B) ratioHigh sensitivity and stabilityFast results in 4 hoursCHIP included as a positive controlOnly E3 is needed from usersRELAYTR FRET Assay - CHIP autoubiquitination DUB Research & Drug DiscoveryReagents30+ recombinant DUBsFluorogenic substrates – Ub-AMC, Ub-Rho110Polyubiquitin chains – eight linkages availableUbiquitin derivatives – catalytic cysteine labeling KitsDiubiquitin explorer kitDUB identification kitDUB enzyme set and profiling kitRELAYTR FRET deubiquitinase assay kit (see schematic below)CRO ServicesDUB profilingTR-FRET DUB activity assay developmentRecombinant DUB production Proteasome Research & CROReagentsHighly purified 19S, 20S and 26S proteasomesFluorogenic substrates – peptidase activitiesAntibodies recognizing proteasomal subunitsKitsPurification of endogenous 26S proteasomeAssaying proteasomal activities...
UBPBio JavaScript seem to be disabled in your browser. You must have JavaScript enabled in your browser to utilize the functionality of this website. Magento Commerce My Account Log In My Cart Home ProductsRELAY TR-FRET Reagents & KitsProteasomes (Proteins/Kits/Antibodies)E1 Activating EnzymesE2 Conjugating EnzymesE3 Ubiquitin LigasesUbiquitin / Sumo ChainsUbiquitin / Ubiquitin-like Proteins / MutantsDeubiquitinating EnzymesInhibitorsFluorescent Peptide / Protein SubstratesUbiquitin Binding ProteinsUbiquitin / UBL DerivativesApoptosis / NecrosisAntibodiesTag/Loading ControlProteasomesE1 / E1-like ProteinsUb / Ub-like ProteinsE3 Ub LigasesDUBsInflammasomesE2 Ub-Conjugating EnzymesAntibody Sampler KitsKitsProtein Expression / Purification ReagentsDNA / Protein LaddersCovid-19 Research Services Service/Tech Support Order Center Distributors Resources Search: ProductsRELAYTR FRET Reagents & KitsProteasomes / Activators / SubunitsE1 Activating EnzymesE2 Conjugating Enzymes E3 Ubiquitin Ligases Polyubiquitin / PolySUMO ChainsUbiquitin / Ubiquitin-like Proteins / MutantsDeubiquitinating EnzymesInhibitors Fluorescent Peptide / Protein SubstratesUbiquitin Binding ProteinsUbiquitin / UBL DerivativesApoptosis / Necrosis AntibodiesKitsProtein Expression / Purification ReagentsDNA / Protein Ladders Covid-19 ResearchCustom Research ServicesOur custom research services are supported by extensive technical expertise and hands-on experience.Protein Production Recombinant protein expression in E. coli, insect, or mammalian systems, followed by multi-step purification to achieve high purity and functional integrity.TR-FRET Reagents, Assays, and Research Services Development and execution of High-throughput TR-FRET–based assays using covalent modifiers compatible with terbium/fluorescein and europium/Cy5 donor–acceptor pairs. Custom Ubiquitin Research Tailored biochemical studies designed to interrogate the ubiquitin-proteasome system, compound screening, and drug target validation.Submit service request with a brief description of your project objectives and technical requirements. RELAYTR FRET PlatformFluorescence Resonance Energy Transfer (FRET) is a powerful technique used to detect molecular proximity between a donor and an acceptor fluorophore within a distance of 1–10 nm. In time-resolved FRET (TR-FRET) assays, the long fluorescence lifetime of lanthanides (such as terbium and europium) enables delayed signal detection, effectively eliminating short-lived background fluorescence. This time-resolved measurement significantly enhances assay sensitivity and specificity. Our RELAYTR FRET Platform leverages terbium and europium chelate-based donors paired with fluorescein- and Cy5-based acceptors. These fluorophores can be covalently conjugated to targets via free thiol or amine groups, ensuring robust and reliable performance and versative assay formats for detecting molecular interactions, post-translational modifications, enzyme activity, protein abundance, and more.We provide both ready-to-use reagents and customized CRO services to support diverse research and development needs. E2 Screening and Profiling Kits29 recombinant E2 enzymesE2 Screening Kit – gel-based AssayIdentifying E2s for E3sAssessing E2 activityE2 Profiling Kit – RELAYTR FRET AssayMonitoring E2-Ub charging (schematic below)High sensitivity – detecting 10 nM E2 activityHigh signal to background (S/B) ratio Profiling 23 ubiquitin E2sHigh throughput compatibleRELAYTR FRET Assay - E2-Ub ChargingDegrader Drug DiscoveryReagents and KitsTerbium, europium, fluorescein or Cy5 labeled ubiquitin, protein G, and anti-6xHis, GST, HA or DYKDDDDK antibody. View all reagents. CRO ServicesReagent production – terbium/europium/dye labelingAssay development and research serviceUb Ligase Activity Assay KitKey FeaturesDetecting Ub ligase autoubiquitination High throughput TR-FRET assay enabled with terbium-/fluorescein-ubiquitinExcellent signal to background (S/B) ratioHigh sensitivity and stabilityFast results in 4 hoursCHIP included as a positive controlOnly E3 is needed from usersRELAYTR FRET Assay - CHIP autoubiquitination DUB Research & Drug DiscoveryReagents30+ recombinant DUBsFluorogenic substrates – Ub-AMC, Ub-Rho110Polyubiquitin chains – eight linkages availableUbiquitin derivatives – catalytic cysteine labeling KitsDiubiquitin explorer kitDUB identification kitDUB enzyme set and profiling kitRELAYTR FRET deubiquitinase assay kit (see schematic below)CRO ServicesDUB profilingTR-FRET DUB activity assay developmentRecombinant DUB production Proteasome Research & CROReagentsHighly purified 19S, 20S and 26S proteasomesFluorogenic substrates – peptidase activitiesAntibodies recognizing proteasomal subunitsKitsPurification of endogenous 26S proteasomeAssaying proteasomal activities...